Next generation sequencing requires the use of high quality analytes for precision and reproducibility. In the framework of NCT MASTER and DKFZ-HIPO, the Sample Processing Laboratory (SPL) provides expertise in the controlled isolation of DNA, RNA, and proteins from different sources of material such as tissue biopsies, FFPE tissue blocks, blood, and cells. Our service also includes state-of-the-art quality control measures and the transfer of suited samples to the DKFZ Genomics and Proteomics Core Facility (GPCF) for sequencing.
We closely work together with the DKFZ-HIPO project partners on initial extraction testing as well as on protocol adaptations according to specific project requirements. So far, analytes for more than 5500 WES/WGS submissions and almost 2000 RNASeq submissions have been transferred to the GPCF. In addition to our routine sample preparation techniques we are currently developing and optimizing extraction procedures of circulating cell-free tumor DNA from liquid biopsies as well as extraction automation processes.
With its central position, the SPL plays a key role for the success of the DKFZ-HIPO projects. In the future, our goal is to provide a service framework for the highthroughput routine analysis of every patient at NCT.